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- W2364275680 abstract "Objective To prepare a highly purified 125 I -pegylated thymosin α1 (PEG-TA1) with satisfied bioactivity for using in the distribution and excretion study in animals. Methods Tyr-PEG-TA1 was iodinated by means of Iodogen method. The ZipTip pipette tips were utilized to monitor the reaction process for the optimization of reaction conditions. A two-step gel filtration chromatography with Sephadex G50 followed by Sephadex G10 was applied to purify the labelled products. The bioactivity assay of PEG-TA1, Tyr-PEG-TA1 and 125 I -Tyr-PEG-TA1 were performed by an enzyme immunoassay (EIA) method. Results The optimum condition of labelling reaction was: shaking for 20 min at 25℃ in 0.1 mol/L phosphate buffer (0.1mol/L NaCl) with the pH value of 7.0. Slight but no significant difference of bioactivity among the PEG-TA1, Tyr-PEG-TA1 and the 125 I -Tyr-PEG-TA1 was observed. After the two-step purification process, a product of 125 I -Tyr-PEG-TA1 with radiochemical purity of 95% and specific activity of 39.4 Bq/ng was obtained. Conclusion A successful preparation of 125 I -Tyr-PEG-TA1 was achieved, and the radiochemical purity and the specific activity of the ~(125)I labeled molecules satisfied the requirements of non-clinical pharmacokinetic study.;" @default.
- W2364275680 created "2016-06-24" @default.
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- W2364275680 date "2005-01-01" @default.
- W2364275680 modified "2023-09-23" @default.
- W2364275680 title "Preparation and purification of ~(125)I-labelled PEGylated thymosin α1" @default.
- W2364275680 hasPublicationYear "2005" @default.
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