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- W2364277110 abstract "Objectives:To detect the expression of melanoma antigen-3(MAGE-3) in human stomach neoplasms and explore the possibility of applying the antigen as target for immunotherapy of stomach neoplasms.Methods:Expression of MAGE-3 gene in 40 stomach neoplasms,para-tumorous mucosa,and 20 samples of chronic gastritis were examined by reverse transcription-polymerase chain reaction(RT-PCR).The samples selected randomly from each gene with positive PCR were confirmed by DNA sequence analysis.Results:In 40 stomach neoplasms,MAGE-3 gene was expressed in 27 tumor tissues(67.5%).The positive rates of MAGE-3 gene expression in neoplasm tissues were significant higher than those of para-tumorous mucosa(15%) and 20 samples of chronic gastritis(10%).The DNA sequence confirmed that the RT-PCR products was truly target cDNA.The expression of MAGE-3 gene in stomach neoplasm tissues was relevant to the intruded depth of neoplasm cells(P0.05),but not relevant to pathological staging,the differentiatation degree of neoplasm cells and lymph node metastasis(P0.05).Conclusion:MAGE-3 gene is expressed in stomach neoplasm at a high frequency,which suggests the possibility of using them as targets for antigen-specific immunotherapy." @default.
- W2364277110 created "2016-06-24" @default.
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- W2364277110 date "2007-01-01" @default.
- W2364277110 modified "2023-09-24" @default.
- W2364277110 title "Expression of melanoma antigen gene-3 (MAGE-3) in gastric neoplasm and its clinical significance" @default.
- W2364277110 hasPublicationYear "2007" @default.
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