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- W2364277556 abstract "Objective To screen tumor susceptibility gene of lung cancer induced by benzo[a]pyrene dihydrodiol epoxide(BPDE) interaction with mice lung DNA and settle a foundation for exploring carcinogenesis mechanism of BPDE.MethodsAmplified fragment length polymorohism(AFLP) combination with immunomagnetic beads isolation were used to enrich and amplify fragments of BPDE interaction with DNA.Differential fragments were displayed by polyacrylamide gel electrophoresis and silver nitrate staining.The differential fragments were sequenced and compared with known genes in genbank.Results 9 differential bands were successfully collectad,cloned and sequenced,and one fragment could not be found in the known genes,and then was submitted to genbank(accession number:EF 176681).Conclusion AFLP combination with immunomagnetic beads isolation technique can be used to screen tumor susceptibility gene of chemaicl carcinogens BPDE,the 9 sequences may be relevant to lung cancer induced by BPDE." @default.
- W2364277556 created "2016-06-24" @default.
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- W2364277556 date "2008-01-01" @default.
- W2364277556 modified "2023-09-27" @default.
- W2364277556 title "Screening of lung tumor susceptibility gene caused by benzo(a) pyrene dihydrodiol epoxide" @default.
- W2364277556 hasPublicationYear "2008" @default.
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