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- W2364280489 abstract "Polymorphism of Ganoderma lucidum monokaryon strains were studied so as to provide molecular basis for the selection of breeding material.Thirty-six monokaryons were developed from strain G0130 of G.lucidum by protoplast monokaryonization.12 SRAP primers,13 ISSR primers and 13 RAPD primers were screened,which could differentiate monokaryons effectively.A total of 320 fragments were amplified,among them 265(accounting for 82.81%of the total)were polymorphic.Genetic similarity coefficient matrix(GS) among thirty-six strains ranged from 0.3741 for the lowest similarity coefficient to 0.9852 for the greatest extent of similarity.The SRAP,ISSR and RAPD dendrogram based on the similarity coefficient matrix was constructed by using NTsys 2.10 e software.Tested strains could be classified into 2 major groups:130-29,130-33 and other five monokaryons fell into same group,while the original strain and the rest of monokaryons fell into the other group.Monokaryons were not classified strictly based on mating types.Comparing with the original strain,monokaryons showed all or part of bands and also the band of original strain not had.The results showed that polymorphism of Ganoderma lucidum monokaryon strains by SRAP,ISSR and RAPD analysis were viable,it could provide reference for mating type identification and the screening of breeding material,reduce the workload and the risk of breeding." @default.
- W2364280489 created "2016-06-24" @default.
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- W2364280489 date "2014-01-01" @default.
- W2364280489 modified "2023-09-25" @default.
- W2364280489 title "Polymorphism of monokaryons of Ganoderma lucidum G0130 based on SRAP,ISSR and RAPD analysis" @default.
- W2364280489 hasPublicationYear "2014" @default.
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