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- W2364283375 abstract "Objective To construct E.coli mutants knockouted the gene encoding high pathogenicity island(HPI) in order to evaluate the function of HPI.Method First,PCR products were obtained by using primers with 50 bp extension which were homologous to HPI and by using template plasmid named pKD3 carrying chloramphenicol resistance gene flanked by FRT sites.Then the products were reformed by the program with the pKD46 recombination system replacing high pathogenicity island in the chromosome of E.coli ZL.Secondy,the strains expressing chloramphenicol resistance gene were selected by chloramphenicol agar.The chloramphenicol resistance gene was then eliminated by using a helper plasmid pCP20.Finally,the purpose gene was identificated and sequenced.Result The constructed mutants were consistent with the expectations.Conclusion The construction of the mutants deleted HPI in chromosome of Escherichia coli was completed successfully." @default.
- W2364283375 created "2016-06-24" @default.
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- W2364283375 date "2011-01-01" @default.
- W2364283375 modified "2023-09-24" @default.
- W2364283375 title "Deletion of high pathogenicity island in chromosome of APEC by red recombination" @default.
- W2364283375 hasPublicationYear "2011" @default.
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