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- W2364284953 abstract "The conserved sequence of soybean genome,higher frequency fragments of DNA bases sequences in genome survey sequence database of soybean were searched by visual basic application programmers,and the basic traits of the conserved sequences were analyzed. On this base,a new molecular marker,named asintegrative conserved sequence amplified polymorphism(ICSAP) was designed. The analysis indicated the more bases of the conserved sequences would follow with less varieties and frequency. The GC content of conserved sequences was closely related to the length of the sequences,because the GC content of conserved sequences stared to increase and remained stable then began to decline as number of bases from 10 to 27. The properties of conserved sequences showed the probability ofAorTbeing increased with proximity to 3'end of DNA,and there are distinct evolutionary relations between the different length conserved sequences,due to the nonrandom distribution of the bases in DNA chains. A total of 886 prime pairs were developed,and the primers were spliced from 10 bases conserved sequences and 8 bases filling sequences. The new molecular marker was better theoretically than prevalent molecular markers in polymorphism of electrophoresis bands and consistency of the primers. The purpose of the analysis was to offer a new idea for searching genomic conserved sequences and molecular evolution of DNA. The new molecular marker may be applied to germplasm identification and map-based cloning of soybean,and what's more,the study methods can be extended to any other organisms." @default.
- W2364284953 created "2016-06-24" @default.
- W2364284953 creator A5026865643 @default.
- W2364284953 date "2013-01-01" @default.
- W2364284953 modified "2023-09-23" @default.
- W2364284953 title "Conserved Sequence of Soybean Genome and Design of Integrative Conserved Sequence Amplified Polymorphism(ICSAP)" @default.
- W2364284953 hasPublicationYear "2013" @default.
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