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- W2364291383 abstract "Diarrhea outbreaks occurred in piglets in early 2013 in Shanghai. To identify the etiological agents, nine fecal samples were randomly collected from ill animals and tested in RT-PCR for the presence of rotaviruses, Transmissible gastroenteritis virus(TGEV)and Porcine epidmic diarrhea virus(PEDV). Four of 9 samples were rotavirus positive(44.4%). All samples were TGEV or PEDV negative. Based on sequences of VP6 and VP7 of reference strain in GenBank, two pairs of primers were designed for amplifying the target genes. The full-lengths of VP6 and VP7 were 1300 bp and 1100 bp. Sequence alignments showed that VP6 shared 64.1%- 87.0% amino acid homology with OSU strain in group A and 26.3%- 60.8% with Gottfriend strain. As for VP7, homology of amino acid sequences ranged from 71.8% to 97.3% with G type strains. Analysis of PoRV VP7 suggested that it belong to G9 genotype. These results established a reliable basis for prevention and control of piglet diarrhea." @default.
- W2364291383 created "2016-06-24" @default.
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- W2364291383 date "2014-01-01" @default.
- W2364291383 modified "2023-09-28" @default.
- W2364291383 title "GENETIC ANALYSIS OF PORCINE ROTAVIRUS INFECTION IN PIGLETS" @default.
- W2364291383 hasPublicationYear "2014" @default.
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