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- W2364294479 abstract "Sequence complexities of polysom-al poly(A)~+mRNA of mouse ascites hepatoma cells were studied in com-parison with normal liver cells by mo-lecular nucleic acid hybridization me-thod.Using single copy DNA or cDNA of poly(A)~+mRNA as probes,satura-tion and kinetic analyses were carried out. In DNA-RNA excess saturation hybridization,polysomal poly (A)~+m-RNA of liver and ascites hepatoma cells hybridized to 1.15% and 2.15% of the ~(125)I-labelled scDNA probe from mouse liver.This accounted for 1.61 % and 3.01% of the mouse genomic expression respectively.The driver pro-portion of poly (A)~+mRNA population in liver cells was 23.2% and that in ascites hepatoma cells was 40.8%.The average copy frequency per each m-RNA per cell was calculated to be nearly one in both mRNA popula-tions. In kinetic hybridization between poly (A)~+mRNA and its complementaryDNA (~3H-cDNA),we analysed the es-quence complexities and frequency dis-tribution of poly (A)~+mRNA popula-tion in two kinds of cells.The hybri-dization rate in liver cells was 71.2% and that in ascites hepatoma cells was 56%.The expressed proportion of mouse genome calculated was 2.6% in liver cells and 4.2 in ascites cells.The cross hybridization between hepatoma poly (A)~+mRNA and liver cDNA of poly (A)~+mRNA or vice versa showed that the difference of the sequence comple-xities between polysomaI poly (A)~+m-RNA population in liver and ascites hepatoma cells was more than 20%. Our data from hybridization ex-periments indicate that the degree of expression of mouse genome is about one fold higher in hepatoma cells than in normal liver cells.The functional meaning of this increase in hepatoma genomic expression and some aspects of nucleic acid hybridization technique are discussed." @default.
- W2364294479 created "2016-06-24" @default.
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- W2364294479 date "1982-01-01" @default.
- W2364294479 modified "2023-09-25" @default.
- W2364294479 title "SEQUENCE COMPLEXITY OF POLYSOMAL POLY(A)~+ mRNA OF MOUSE ASCITES CELLS" @default.
- W2364294479 hasPublicationYear "1982" @default.
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