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- W2364295079 abstract "West Nile virus(WNV) is by far the most widely distributed arbovirus.The WNV infection has been recognized as an emerging infectious disease with possible transmitting risk in our country.The pairs of primers and TaqMan probes were designed and synthesized based on the nucleotide sequences of WNV in GenBank.After optimization,the real-time PCR was established.These primers were applied to detect the nucleic acids of WNV,Japanese B encephalitis virus(JEV) and Dengue viruses(DEN),and the latter two viruses could not be detected.Compared with conventional RT-PCR,real-time PCR appeared to be approximately 100 times more sensitive in detection of WNV.It suggests that the real-time PCR developed for the detection of WNV shows high degree of specificity and sensitivity,and can be used for WNV inspection and investigation." @default.
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- W2364295079 date "2012-01-01" @default.
- W2364295079 modified "2023-09-23" @default.
- W2364295079 title "Detection of West Nile virus by real-time RT-PCR" @default.
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