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- W2364302008 abstract "The extraction and the detection of cell-associated polyvinyl alcohol dehydrogenase(PVADH) were studied.Results showed that PVADH activity reached the maximum as extracted by non ionic surfactant Triton X-100,which were 246.5% and 831.3% higher than those of ionic surfactant sodium alkyl benzene sulfonate(LAS) and cetylpyridinium bromide(CPB).As for non ionic surfactants,PVADH activity was enhanced by 101.4% compared with that of Tween 80.The concentration and the extraction time obviously affected PVADH activities and the appropriate values were 1% and 18 h,respectively.Under the condition,the maximum PVADH activity of 14.9 U/g was obtained.In PVADH detection system,PVADH activity determined by using the electron acceptors as the starter was enhanced by 60.6% and 126.5% compared with that of enzyme solutions and the substrate.It was very important for the detection of PVADH activity keeping enzyme solutions and pyrroloquinoline quinone(PQQ) at 30 ℃ for 10 min before determination and 59.1% of the activity was increased.The suitable concentrations of KCN,CaCl2 and PQQ were 1.0 mmol/L,0.5 mmol/L and 2 μmol/L,and 37.1%,38.7% and 214.0% of PVADH activity were enhanced with addition of them in the detection system." @default.
- W2364302008 created "2016-06-24" @default.
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- W2364302008 date "2009-01-01" @default.
- W2364302008 modified "2023-09-23" @default.
- W2364302008 title "Extraction and detection of polyvinyl alcohol dehydrogenase" @default.
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