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- W2364313074 abstract "AIM: To optimize the pilot-scale fermentation procedure of E.coli producing tumor targeted human IFN-α2a and to obtain high cell density and high level expression of the aim protein. METHODS: The effects of the rang of pH, dissolved oxygen, culture time, induction time and the manner of feeding were analyzed with 10 L of fermentation tank. RESULTS: Under the established conditions, we performed a three-time repeated fermentation. The final cell density was A 600 nm 25-30 and more than 50 g of wet bacteria per liter could be obtained. The expression level of recombinant protein was higher than 3.2×10 9 U/L. CONCLUSION: The results show that the established fermentation procedure is stable, of short cycle and with high expression, which lays the basis for further purification and large-scale production of IFN-α2a-NGR." @default.
- W2364313074 created "2016-06-24" @default.
- W2364313074 creator A5075378373 @default.
- W2364313074 date "2004-01-01" @default.
- W2364313074 modified "2023-09-22" @default.
- W2364313074 title "High cell density fermentation of engneering E. coli expressing tumor targeted interferon-α2a" @default.
- W2364313074 hasPublicationYear "2004" @default.
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