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- W2364328973 abstract "Objective To develop a new genotyping assay-Tm genotyping assay,using real-time PCR with fluorescence hybridisation probe,for the polymorphisms typing in promoter region of mannose-binding lectin(MBL)gene.Methods Genomic DNA was extracted from peripheral blood of 30 healthy blood donors.Melting curve of target DNA fragments were drawn using real-time PCR through Light Cycler Instrument integrated with specific oligonucleotide probes,and the samples were genotyped according to the peak of the melting temperature(Tm).At last,PCR products was examined by gene sequencing to validate Tm genotyping assay.Results Tm genotyping assay was established successfully.A full cycle of Tm genotyping assay takes only 180 minutes of period.The results of Tm genotyping assay was completely consistent with the sequencing results.Conclusions The Tm genotyping assay in detecting the polymorphisms of MBL gene was rapid,accurate and excellently reproducible with promising clinical applications." @default.
- W2364328973 created "2016-06-24" @default.
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- W2364328973 date "2008-01-01" @default.
- W2364328973 modified "2023-09-23" @default.
- W2364328973 title "Establishment of rapid genotyping of polymorphisms in promoter region of mannose-binding lectin gene using real-time PCR with fluorescent hybridisation probes" @default.
- W2364328973 hasPublicationYear "2008" @default.
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