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- W2364331808 abstract "Five extraction methods including Takara RNA,Tiangen kit,Invitrogen kit,modified CTAB-LiCl and CTAB-isopropyl alcohol method were compared for extracting total RNA from the leaves of four mangrove plants(Bruguiera gymnorrhiza,Kandelia candel,Aegiceras corniculatum,and Aricennia marina).The five methods were all suitable for total RNA extraction in leaves of A. marina.Tiangen kit was successfully used to extract the RNA in leaves of A.corniculatum,A.marina,and K.candel,but failed to extract the RNA in leaves of B.gymnorrihiza.Invitrogen kit extracted high quality RNA of 5.gymnorrihiza and K.candel,but could not remove the protein of leaves of A.corniculatum.The RNA amount extracted using CTAB-LiCl and improved CTAB-isopropyl alcohol methods was lower than using RNA extraction kits;besides,the efficiency of reverse transcription PCR of CTAB-LiCl and CTAB-isopropyl alcohol methods was lower." @default.
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- W2364331808 date "2011-01-01" @default.
- W2364331808 modified "2023-09-27" @default.
- W2364331808 title "Analysis and improvement of high-quality RNA extraction in leaves of mangrove plants" @default.
- W2364331808 hasPublicationYear "2011" @default.
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