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- W2364338514 abstract "Objective To investigate the differentiation of dental pulp cells into odontoblast-like cells correlates with the changes of cells proliferation.Methods Human dental pulp cells were cultured by tissue enzymatic digestion method and identified by vimentin and keratin antibody.Addition of βGP to the culture medium induced odontoblast features in the cultured pulp cells.Light microscopy and von Kossa staining were applied to observe the morphology and nodel formation of odontoblasts like cells.Flow cytometry were used to detect the changes of cells proliferation during dental pulp cell differentiation.Results Cultured cells were all vimentin positive and keratin negative.At the second week,cells were stratified,then they polarized and some of them exhibited a typical cellular extension at 3rd week.von Kossa staining became positive at the last week.Flow cytometry demonstrated cells proliferation was suppressed,most cells are at the G0/G1 stage.Conclusion Dental pulp cells successfully differentiated into odontoblast-like cells in vitro." @default.
- W2364338514 created "2016-06-24" @default.
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- W2364338514 date "2008-01-01" @default.
- W2364338514 modified "2023-09-26" @default.
- W2364338514 title "Changes of Cell Cycle During Dental Pulp Cell Differentiation" @default.
- W2364338514 hasPublicationYear "2008" @default.
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