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- W2364351519 abstract "To separate and purify the Nattokinase and to prepare antiserum against Nattokinase.Nattokinase was purified through procedures including ammonium sulfate differential precipitation,sephadexG-100,and CM-Sephrose-6B FF chromatography,and was detected on gels of SDS-PAGE.Rabbit antiserum against Nattokinase was prepared by muscle immunization mixed with Freund's adjuvant and the titer was detected by double immunodiffusion carried out on agar gel.The purified sample's specific activity was 608.6 UKu·mg-1 protein and was purified by 28.4 fold.The purified nattokinase showed single protein band on gels of SDS-PAGE.The titer of rabbit antiserum against Nattokinase was 1∶64.The rabbit antiserum against Nattokinase is prepared successfully,and it provides the material foundation to the function study of Nattokinase." @default.
- W2364351519 created "2016-06-24" @default.
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- W2364351519 date "2009-01-01" @default.
- W2364351519 modified "2023-09-28" @default.
- W2364351519 title "Isolation and Purification of Nattokinase and Preparation of Rabbit Antiserum Against It" @default.
- W2364351519 hasPublicationYear "2009" @default.
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