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• W2364353970 abstract "Objective To assess the quality of HCV RNA RT-PCR dete ction in clinical laboratories of China from the year 2000 to 2002. Methods There were two times external quality assessment (EQA) for HCV RNA RT-PCR detection each year. A panel consisting of five lyophilized plasma samples including three to four positive were distributed. The concentrations of HCV RNA positive samples were within 10 4 and 10 7 copies/ml. The participants of the EQA program were required to detect the samples and return the data. All date were statistical analysed by the computer. Results The ratio of the participants who got correct qualitative detection results from all samples within a panel increased from 53 3%(24/45)(001) and 4 8%(2/42)(002) in the year 2000, 73 8%(31/42) (011) and 79 2%(42/53)(012) in the year 2001 to 89 7%(105/117) (021) and 91 6%(109/119)(022) in the year 2002 The ratio of false positive declined from 26 7% in 2000, 14 3% in 2001 to less than 8% in 2002. For samples with 10 5 and 10 4 copies/ml, the ratio of false negative also decreased from 15 6%(sample 0011), 19 0%(sample 0025)and 81 0%(sample 0023)in 2000, to 11 9% (sample 0112) , 11 3%(0125)and 9 5%(0115) in 2001. in 2002, the ratio of false negative markedly declined to 4 6%(sample 0221)、3 7%(sample 0223)and 3 8%(sample 0212). From the information provided, we can get an impression that there are significantly improvement in specificity, sensitivity and efficiency of the RT-PCR kits that were used by participants from 2000 to 2002. Conclusion There is a good tendency for the quality of HCV RNA RT-PCR detection from 2000 to 2002 in clinical laboratories of China. The ratio of false positive and false negative declined significantly in clinical laboratories of China. The ratio of false positive and false negtive declined significantly in the year 2002 means that the EQA program can monitor the quality problem existed in clinical PCR laboratories, and improve the detection quality of the laboratories." @default.
• W2364353970 created "2016-06-24" @default.
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• W2364353970 date "2003-01-01" @default.
• W2364353970 modified "2023-09-28" @default.
• W2364353970 title "External quality assessment for detection of hepatitis Cvirus RNA by RT-PCR in clinical laboratories of China" @default.
• W2364353970 hasPublicationYear "2003" @default.
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