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- W2364355862 abstract "Objective To investigate the mutation of embB and pncA genes in mycobacterium tuberculosis isolates, and assess its clinical value. Methods The drug-sensitivity of 106 clinical isolates of mycobacterial species was identified by the tranditional method, and then analyzed the mutation of their embB and pncA genes with PCR-SSCP. Mycobacterium tuberculosis strain H 37 RV was used as a control. Results The mutational frequency of pncA gene in 94 PZA-resistant mycobacterium tuberculosis was 44 6%(42/94). The mutational frequency of embB gene in 83 EMB-resistant isolates was 54 2%. The frequency of both genes mutation was 11 8%(11/94). Conclusion EMB and PZA resistances in some mycobacterium tuberculosis isolates were due to mutations of embB and pncA genes. PCR- SSCP method might become a simple and rapid diagnostic test for genotypes of EMB and PZA resistant mycobacterium tuberculosis." @default.
- W2364355862 created "2016-06-24" @default.
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- W2364355862 date "2004-01-01" @default.
- W2364355862 modified "2023-09-24" @default.
- W2364355862 title "The Mutation of embB and pncA Genes in Drug-resistanct Mycobacterium Tuberculosis and Its Clinical Value" @default.
- W2364355862 hasPublicationYear "2004" @default.
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