Matches in SemOpenAlex for { <https://semopenalex.org/work/W2364359567> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W2364359567 abstract "The phylogenetic relationships of 5 species of snappers (Lutjanus stellatus, L.sebae,L.vitta, L.fulvus and L.fulviflamma) were studied comparatively, which were based on 2 methods: restriction fragment length polymorphism of mitchondrial DNA (mtDNA-RFLP), RFLP of cytochrome b (Cyt b) gene segment(900bp) amplified by polymerase chain reaction(PCR-RFLP). The sample numbers of Lutjanus stellatus, L.sebae, L.vitta, L.fulvus and L.fulviflamma were 17,17,35,17 and 17, respectively, which are collected in the South China Sea from Sep.2002 to Jun.2003. The mtDNA being isolated and purified from fresh livers of samples range from 17.05±0.46 to 17.77±0.91 kb. Having been digested by 19 restriction endonucleases recognized 6 base pairs(EcoRⅠ, HindⅢ, BglⅠ, BglⅡ, StyⅠ, XbaⅠ, DraⅠ, PstⅠ, PvuⅡ, XhoⅠ, BamHⅠ, ScaⅠ, EcoRⅤ,SmaⅠ, MluⅠ, SalⅠ, HinfⅠ, KpnⅠ,HpaⅠ), the mtDNA were analysed. The result of mtDNA-RFLP shows that L.vitta could be divided into HE and HD, based on the color of stripe on the middle of the side. The physical maps of mtDNA from L.stllatus and L.sebae have been constructed based on the endonucleases that are chosen from 19 restriction endonucleases.By comparison of the sequence of cytochrome b gene PCR-RFLP using different amplification templates (one from purified mtDNA and the other from nDNA), the possible interferences of nuclear mitochondrial pseudogenes (Numts) were excluded and the possible pseudogenes from nDNA were preliminarily discussed. Having been digested by 6 restriction endonucleases recognized 4 base pairs(StyⅠ, HinfⅠ, HpaⅡ, MspⅠ, Sau96Ⅰ, SinⅠ, TaqⅠ, HaeⅡ), the sequences were analysed. The results of mtDNA PCR-RFLP support that L.vitta could be divided into two species: HE and HD by the 0.0751 of divergency index. The results reveal that all of the 2 analysis methods are useful for seeking out the genetic markers among species effectively. And the results from RFLP indicate that the L.vitta could be divided into two species based on the color of stripe on the middle of the side. At the same time, the constructions of phylogenic trees by neighbor-joining(NJ) method show that there are some differences along different methods on the specific level when they are used for phylogenesis." @default.
- W2364359567 created "2016-06-24" @default.
- W2364359567 creator A5035386182 @default.
- W2364359567 date "2005-01-01" @default.
- W2364359567 modified "2023-09-23" @default.
- W2364359567 title "Comparison on RFLP of mitochondrial DNA and Cyt bgene segment for 5 species in Lutjanus" @default.
- W2364359567 hasPublicationYear "2005" @default.
- W2364359567 type Work @default.
- W2364359567 sameAs 2364359567 @default.
- W2364359567 citedByCount "0" @default.
- W2364359567 crossrefType "journal-article" @default.
- W2364359567 hasAuthorship W2364359567A5035386182 @default.
- W2364359567 hasConcept C104317684 @default.
- W2364359567 hasConcept C128319531 @default.
- W2364359567 hasConcept C141231307 @default.
- W2364359567 hasConcept C148292235 @default.
- W2364359567 hasConcept C151292725 @default.
- W2364359567 hasConcept C153911025 @default.
- W2364359567 hasConcept C168007829 @default.
- W2364359567 hasConcept C193252679 @default.
- W2364359567 hasConcept C24586158 @default.
- W2364359567 hasConcept C34957205 @default.
- W2364359567 hasConcept C49105822 @default.
- W2364359567 hasConcept C54355233 @default.
- W2364359567 hasConcept C84289048 @default.
- W2364359567 hasConcept C86803240 @default.
- W2364359567 hasConceptScore W2364359567C104317684 @default.
- W2364359567 hasConceptScore W2364359567C128319531 @default.
- W2364359567 hasConceptScore W2364359567C141231307 @default.
- W2364359567 hasConceptScore W2364359567C148292235 @default.
- W2364359567 hasConceptScore W2364359567C151292725 @default.
- W2364359567 hasConceptScore W2364359567C153911025 @default.
- W2364359567 hasConceptScore W2364359567C168007829 @default.
- W2364359567 hasConceptScore W2364359567C193252679 @default.
- W2364359567 hasConceptScore W2364359567C24586158 @default.
- W2364359567 hasConceptScore W2364359567C34957205 @default.
- W2364359567 hasConceptScore W2364359567C49105822 @default.
- W2364359567 hasConceptScore W2364359567C54355233 @default.
- W2364359567 hasConceptScore W2364359567C84289048 @default.
- W2364359567 hasConceptScore W2364359567C86803240 @default.
- W2364359567 hasLocation W23643595671 @default.
- W2364359567 hasOpenAccess W2364359567 @default.
- W2364359567 hasPrimaryLocation W23643595671 @default.
- W2364359567 hasRelatedWork W1825384920 @default.
- W2364359567 hasRelatedWork W2010455074 @default.
- W2364359567 hasRelatedWork W2026926527 @default.
- W2364359567 hasRelatedWork W2090973544 @default.
- W2364359567 hasRelatedWork W2149109737 @default.
- W2364359567 hasRelatedWork W2169876369 @default.
- W2364359567 hasRelatedWork W2192938586 @default.
- W2364359567 hasRelatedWork W2268268104 @default.
- W2364359567 hasRelatedWork W2282370393 @default.
- W2364359567 hasRelatedWork W2326187243 @default.
- W2364359567 hasRelatedWork W2416963348 @default.
- W2364359567 hasRelatedWork W2433653952 @default.
- W2364359567 hasRelatedWork W2558301193 @default.
- W2364359567 hasRelatedWork W2589213048 @default.
- W2364359567 hasRelatedWork W2618405726 @default.
- W2364359567 hasRelatedWork W2728850037 @default.
- W2364359567 hasRelatedWork W2737221872 @default.
- W2364359567 hasRelatedWork W2773260746 @default.
- W2364359567 hasRelatedWork W2337844952 @default.
- W2364359567 hasRelatedWork W2339322376 @default.
- W2364359567 isParatext "false" @default.
- W2364359567 isRetracted "false" @default.
- W2364359567 magId "2364359567" @default.
- W2364359567 workType "article" @default.