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- W2364376291 abstract "A novel immunosensor for rapid separation-free determination of α-fetoprotein antigen(AFP)in human serum is proposed.The immunosensor is prepared by co-immobilizing AFP antibody(Ab-AFP)and mercapto succinic amide copper(II)(CuL)on a glassy carbon electrode(GCE)through titania sol-gel film by vapor deposition method(GCE|Ab-AFP/TiO2/CuL).The electrochemical behavior of the immobilized CuL displays a surface-controlled electrode process.CuL can be used as an electro-reductive catalyzation for H2O2.After the immunosensor is incubated with AFP solution at 25℃ for 30 min,the access of activity center of CuL is partly inhibited,which leads to a linear decrease of the catalytic efficiency of CuL by H2O2 in two AFP concentration ranges from 0.5 to 3.9 ng/mL and 3.9 to 100 ng/mL.Under optimal conditions the detection limit for AFP immunoassay is 0.081 ng/mL(3σ).The immunosensor shows a good accuracy and acceptable storage stability,precision and reproducibility.This method decreases the detection cost and shortens the analytical time,thus would be valuable for clinical immunoassay." @default.
- W2364376291 created "2016-06-24" @default.
- W2364376291 creator A5065058975 @default.
- W2364376291 date "2008-01-01" @default.
- W2364376291 modified "2023-09-23" @default.
- W2364376291 title "Electrochemical Immunosensor for α-Fetoprotein Antigen Based in Human Serum on α-Fetoprotein Antibody and Mercapto Succinic Amide Copper Coimmobilized Electrode" @default.
- W2364376291 hasPublicationYear "2008" @default.
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