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- W2364378419 abstract "Objective To test the feasibility of applying laser capture microdissection(LCM) combined with microarray technique in studies on expression of sensory neuron genes in dorsal root ganglion(DRG) of rats. Methods Sensory neurons were obtained from frozen sections of rat L4-L6 DRGs by using LCM. The quality and yield of total RNA extracted from captured cells were tested in order to determine the impact of frozen-section cutting, staining and capture procedure. Part of the array results was verified by real-time RT-PCR. Results No degradation was detected, and via a single cycle of T7 linear amplification, the yield was sufficient for RT-PCR and microarray analysis. Conclusion Combined with effective RNA protection and linear amplification, LCM can be applied for gene transcriptional analysis of DRG sensory neurons." @default.
- W2364378419 created "2016-06-24" @default.
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- W2364378419 date "2009-01-01" @default.
- W2364378419 modified "2023-09-23" @default.
- W2364378419 title "Combining Laser Capture Microdissection and Microarray to Analyze the Gene Expression of Rat Dorsal Root Ganglion Sensory Neurons" @default.
- W2364378419 hasPublicationYear "2009" @default.
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