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- W2364383191 abstract "The culture condition for producing β-cyclodextrin glucanotransferase by E.coli BL21(DE3) was primarily established by orthogonal experimental design.The results showed that the optimal fermentation conditions were as follows: the inoculum size was 1%;The load of 100ml-flask was 15ml;initial pH value was 7.0;the concentration of Ca2+was 1.25mmol/L,the concentration of Mg2+was 2.50mmol/L,incubation temperature was 37℃ and the speed was 150r/min.When the value of OD600reached 1.4,α-lactose was added so as to the final concentration to 5.0g/L,the speed was increased to 170r/min,and induced 12h at 37℃ then filtered,the β-CGTase activity reached the maximum value as 16.3μ/ml." @default.
- W2364383191 created "2016-06-24" @default.
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- W2364383191 date "2013-01-01" @default.
- W2364383191 modified "2023-09-26" @default.
- W2364383191 title "Optimization of the culture conditions of E.coli BL21(DE3) producing β-cyclodextrin glucanotransferase" @default.
- W2364383191 hasPublicationYear "2013" @default.
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