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- W2364393448 abstract "Objective: To join different DNA fragments and clone the joint DNA.Methods: We randomly ligated the blunt-ended DNA fragments with T4 DNA ligase,cleaved the ligated molecules with the restriction endonucleases,then separated the hydrolysed products by agarose gel electrophoresis.We identified the specific band on the agarose gel,from which the DNA molecules were recovered,integrated into the linearized vector plasmid and transformed into the competent E.coli cells.Results: In this way we successfully joined the different DNA fragments and constructed the recombinant plasmids haboring the joint DNA.Conclusion: The stragety is simple,convenient and reliable,providing an alternative procedure for joining and cloning DNA which can be used in molecular biology research and gene engenering." @default.
- W2364393448 created "2016-06-24" @default.
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- W2364393448 date "2012-01-01" @default.
- W2364393448 modified "2023-09-25" @default.
- W2364393448 title "Construction of Recombinant Plasmids by Random Ligation of Blunt-Ended DNA" @default.
- W2364393448 hasPublicationYear "2012" @default.
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