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- W2364400368 abstract "Objective To investigate the expression of LacZ gene in Schwann cells(SCs) introduced by adenoviral vector in vitro. Methods The primary culture and purification of SCs was established and the efficiency of transfection of Ad-LacZ was determined by X-gal histochemical staining in SCs. Results Ad-LacZ infected SCs in a dose-dependant and time-dependant manner. As MOI (multiplicity of infection) increased, there were increasing numbers of infected cells, so that nearly 100% of the cells were blue following X-gal staining at an MOI of 200 twenty-four hours after infection. As time period of infection prolonged, there were also increasing numbers of infected cells, so that nearly all the cells were blue at an MOI of 100 forty-eight hours after infection. The MTT test showed no significant difference among the cell groups infected with or without different MOI Ad-LacZ. Conclusions (1)adenoviral vector can transfer LacZ gene into SCs in culture efficiently; (2)the viability of infected SCs was not affected. The results suggest that neurotrophic factors could be introduced into SCs by adenoviral vector to promote peripheral nerve regeneration." @default.
- W2364400368 created "2016-06-24" @default.
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- W2364400368 date "2003-01-01" @default.
- W2364400368 modified "2023-09-26" @default.
- W2364400368 title "Adenoviral vector-mediated transfer of LacZ genes into rat Schwann cells in vitro" @default.
- W2364400368 hasPublicationYear "2003" @default.
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