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- W2364403203 abstract "Objective To investigate the expression of aquaporin-1(AQP-1) in pleural mesothelial cells(PMCs) and the influence of glucose thereupon.Methods Rat PMCs were isolated,cultured,and divided into two groups,ie.a glucose group,cultured with glucose of different concentrations for 24 hours,and a control group,cultured in D-MEM/F-12 medium.The 100 mmol/L glucose group was administered at the time points of 6,12,18,and 24 hours respectively.RT-PCR and Western blotting were used to analyze the mRNA and protein expression of AQP-1.Results The absorbance values of AQP-1 protein expression were 54.02±4.61,127.84±9.41,and 231.62±22.63,respectively in the PMCs treated with glucose of the concentrations of 50,100,and 200 mmol/L,all significantly higher than those in the control group(22.45±2.16,all P0.01).The absorbance values of AQP-1 protein expression were 24.68±2.56,58.68±3.67,89.61±6.62,and 113.41±7.65 in the PMCs treated with glucose of the concentration of 100 mmol/L after 6,12,18,and 24 hours,all significantly higher than those in the control group(11.81±1.45,P0.01).Conclusions Glucose induces the expression of AQP-1 mRNA and protein.AQP-1 participates in the pleural fluid formation." @default.
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- W2364403203 date "2009-01-01" @default.
- W2364403203 modified "2023-09-22" @default.
- W2364403203 title "Effect of Glucose on Expression of Aquaporin-1 in Pleural Mesothelial Cells in Rats" @default.
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