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- W2364408867 abstract "Objective To investigate the expression of ezrin gene in some carcinoma cells,to detect the transcriptional activity of its 5′-flanking region,and to elucidate the transcriptional regulatory mechanism of the ezrin gene in carcinoma cells.Methods Expression of ezrin gene in esophageal carcinoma EC109,EC171,EC8712 and SHEEC,gastric carcinoma N87 and BGC823,lung carcinoma A549 and 95D,hepatocellular carcinoma HepG2,leukemia K562,and cervical carcinoma HeLa cells was detected using reverse transcriptase polymerase chain reaction(RT-PCR) and western blot analysis.Plasmid pGLB-hE(-1444/+134),containing1444/+134 of ezrin gene fused to the luciferase gene,was generated by PCR method.Transcriptional activity of the-1444/+134 region in some carcinoma cells was examined using dual-luciferase reporter assay system.Results All the tested carcinoma cells high-expressed ezrin mRNA and Ezrin protein.-1444/+134 region of ezrin gene had strong transcriptional activity compared with SV40 promoter(P0.05) in EC109,Hela,A549 and BGC823 cells.Conclusion The transcriptional activity of the ezrin gene 5′-flanking region may paly an important role in the high-expression of Ezrin in some carcinoma cells." @default.
- W2364408867 created "2016-06-24" @default.
- W2364408867 creator A5038387396 @default.
- W2364408867 date "2008-01-01" @default.
- W2364408867 modified "2023-09-26" @default.
- W2364408867 title "Expression of ezrin Gene in Some Carcinoma Cells and Identification of Its 5′-Flanking Transcriptional Activity" @default.
- W2364408867 hasPublicationYear "2008" @default.
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