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- W2364420669 abstract "According to the sequence of the accession No.Y10404 of infectious salmon anaemia virus(ISAV),a partial gene fragment was synthesized and inserted into T vector to establish positive quality control and a pair of specific primers was designed and synthesized.The SYBR-GreenⅠ real-time PCR was established by the positive quality control.The reactive system was optimized,its specificity and sensitivity were analyzed and the standard curve was performed.The established assay was applied into the detection of the positive sample.The results showed that the optimized primers' concentration was 0.5umol/L.The assay could detect ISAV specifically and was 1000 fold more sensitive than conventional PCR.And its sensitivity was 9.5×10-8ug/uL.ISAV could be detected from the positive sample,and Tm value was 82.5℃.A standard curve was obtained and had its r20.99 and was highly reliable,so it can be applied into quantitative detection." @default.
- W2364420669 created "2016-06-24" @default.
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- W2364420669 date "2007-01-01" @default.
- W2364420669 modified "2023-09-23" @default.
- W2364420669 title "DETECTION OF INFECTIOUS SALMON ANAEMIA VIRUS WITH SYBR-GREEN I REAL-TIME PCR ASSAY" @default.
- W2364420669 hasPublicationYear "2007" @default.
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