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- W2364440169 abstract "In order to research the effects of sodium butyrate on the expression of PepT1,NHE2 and NHE3 mRNA abundances in primary cultured pig intestinal epithelial cell(IEC) in vitro,newborn pig was used to establish the method for isolation and primary culture of IEC.Four levels of sodium butyrate were added to the media for 96 h,respectively.The target genes mRNA abundances were determined by real-time RT-PCR using SYBR Green I RTPCR mix Kit.Cells were collected for analyzing the PepT1 and NHE2 mRNA abundances by real-time RT-PCR method.The results showed that the expression of PepT1 and NHE2 mRNA abundances was increased in sodium butyrate treatment and exhibiting dosage-depended also.The dosage-depended effect of NHE3 mRNA in sodium butyrate treatment was not significant." @default.
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- W2364440169 date "2010-01-01" @default.
- W2364440169 modified "2023-09-24" @default.
- W2364440169 title "Effects of sodium butyrate on expression of PepT1, NHE2 and NHE3 mRNA abundances in primary cultured IEC in vitro." @default.
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