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• W2364442798 abstract "Studies were conducted to reveal the changes in growth, serum biochemical indices and growth hormone (GH), insulin-like growth factor Ⅰ(IGF-Ⅰ) and insulin (IN) mRNA expression abundance of Nile tilapia (Oreochromis niloticus) during fasting and re-feeding. Nile tilapia with initial body weight (62.50 ± 3.44) g were starved for 28 d and then fed for 21 d under controllable indoor environment. Fish were sam-pled at 0, 7, 14, 21 and 28 d during fasting and at 14 and 21 d during re-feeding, respectively. The results indicated that the body weight was significantly decreased when fasting over 7 d (P0.05), and signifi-cantly increased when re-feeding for 21 d (P0.05). The hepatosomatic index was significantly decreased throughout the experiment (P0.05). Significant reduction was observed in the content of triglyceride and glucose, and in the activities of alkaline phosphatase, glutamic oxaloacetic transaminase and glutamic py-ruvic transaminase after fasting (P0.05); After re-feeding, the value of these indices increased in varying degrees, but the activity of transaminase was significantly lower than initial value(P0.05). There was no change in total cholesterol, high density lipoprotein cholesterol or low density lipoprotein cholesterol dur-ing the experiment (P0.05). Serum GH and liver GH mRNA levels showed significantly up-regulation, whereas significant down-regulation was observed in serum IGF-Ⅰ and liver IGF-Ⅰ mRNA levels after fasting, and after re-feeding both of them increased (P0.05). IN mRNA level was significantly increased during fasting for 7-21 d (P0.05), but its level was not obviously changed when fasting for 28 d (P0.05), then was significantly decreased after re-feeding (P0.05). The present study revealed that fasting could restrain the growth of O. niloticus, promote serum triglyceride and glucose breakdown, and decrease the activity of transaminase. Serum GH/IGF-Ⅰ and liver GH/IGF-Ⅰ mRNA expression abundance displayed synchronous changes and the liver IN mRNA expression abundance was significantly increased when fasting for 7-21 d and then decreased to normal level when fasting for 28 d. It suggests that the level of GH/IGF-Ⅰ gene transcription of O. niloticus may be the most important factor in determining the levels of hormone in serum, while down-regulation of serum insulin may be due to its release reduction in islet when fasting." @default.
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• W2364442798 date "2012-01-01" @default.
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• W2364442798 title "The changes in growth, serum biochemical indices and GH/IGF- Ⅰ/IN mRNA expression abundance ofOreochromis niloticusduring fasting and re-feeding" @default.
• W2364442798 doi "https://doi.org/10.3724/sp.j.1231.2012.27562" @default.
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