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- W2364452017 abstract "Enterokinase plays a key role in the construction of the expression system of artificial fusion protein because of its highly specific cleavage of the pentapeptide recognition sequence (Asp Asp Asp Asp Lys).In this paper,the influence factors for the high level expression of recombinant enterokinase in the Pichia pastoris yeast,such as the optimum temperature (30℃),pH 6.0,methanol volume concentration (1.0%~3.0%),yeast density and so on,were optimized in the Bioflo Ⅲ fermentor.The total enterokinase activity of 58 kIU/L culture broth and yeast dry weight of 150 g/L were obtained from experiments." @default.
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- W2364452017 date "2003-01-01" @default.
- W2364452017 modified "2023-09-25" @default.
- W2364452017 title "The influence factors for the high-level expression of recombinant enterokinase in the Pichia pastoris" @default.
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