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- W2364456380 abstract "Objective : This present study was conducted to examine the effects on in vitro growth of pre-antral mouse follicles by thecal-stromal cells attached to around the follicular membrane in culture medium without hormones. Methods : Pre-antral follicles (100-130 m) used in our studies were isolated mechanically by fine 30 G needles attached to 1 ml insulin syringe from mice ovaries of 20-25 days old female ICR strain. Isolated pre-antral follicles were divided into three groups by attached status of thecal-stromal cell layers to follicular membrane. Follicular Initial diameters of group Ⅰ was 112.5 (mean)6.2 (SD) m (n=31), group Ⅱ was 112.87.8 m (n=23), and group Ⅲ was 115.1 EX>${pm}$6.5 m (n=27). Divided pre-antral follicles were washed in fresh Ham's F-10 medium and cultured in 20 l s of DMEM with glutamine, glucose and pyruvate under mineral oil on the 60 mm culture dish. All experimental media were supplemented with 10% FBS without hormones. Media were exchanged wholly by fresh media every 2 days in culture. Diameters of intrafollicular oocytes and cultured pre-antral follicles were measured using an precalibrated cross ocular micrometer at 200 every day during 6 days in vitro culture. Results were considered statistically significant hen p value was less than 0.05 using Student's t-test. Results : The mean and standard deviation (SD) of initial diameters of pre-antral follicles was 113.52.2 m in three groups. Follicular growth rate of group Ⅲ was significantly (p18.7 m, 196.924.1 m and 284.247.6 m in group Ⅰ, group Ⅱ and group Ⅲ, respectively. Growth rate of intrafollicular oocytes between hree groups were not difference and revealed to continuous growth patterns in whole culture periods. Follicular diameter after culture for 7 days were not measured because of disruption of follicular structure or outgrowth of granulosa cells. Conclusion : Pre-antral follicles were grown very well in DMEM medium without hormones. Thecalstromal cells attached to the surface of follicular membrane has acted effectively in vitro growth of follicles." @default.
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- W2364456380 date "2004-03-01" @default.
- W2364456380 modified "2023-09-25" @default.
- W2364456380 title "Establishment of Culture System for In Vitro Culture of Mouse Pre-antral Follicles: Effect on In Vitro Growth by Thecal-Stromal Cells in Culture Medium without Hormones." @default.
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