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- W2364458077 abstract "Objective:To develop an RP-HPLC method for the determination of tussilagone in the flower buds of Tussilago farfara L.(Flos Farfarae) from crude drug market of eight different areas,nine batches and crude drug of one main production area.Methods:The crude drug was extracted by 95% ethanol after crush under ultrasonic extractor and then analysed.The chromatographic column Dikma DiamonsilTM C18(4.6 mm×250 mm,5 μm) was used with methanol-water(85:15,v/v) as the mobile phase.The flow rate was 1.0 mL·min-1,analytical time was 15 min,UV detection wavelength was set at 220 nm,and injection volume was 20 μL.The analyte was qualitated by retention time and quantitated by peak area using external reference standard calibration curve.Results:The retention time of tussilagone was 12.29 min.There were excellent linearity between peak area and concentration of analyte in the concentration range of 26.5-212 μg·mL-1 for tussilagone,and the correlation coefficient was 0.9997.The limits of detection and quantification were 0.54 and 1.75 μg·mL-1,respectively.The intra-and inter-day RSDs were 0.76%(n=6)and 0.69%(n=4),respectively.The added recoveries(n=6)were 99.35%,106.4% and 95.88%,and RSDs were 3.9%,1.5% and 0.60% at low,medium and high levels,respectively.Conclusion:This method is accurate,quick,simple and reproducible and can be used to determine the content of tussilagone in Flos Farfarae." @default.
- W2364458077 created "2016-06-24" @default.
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- W2364458077 date "2009-01-01" @default.
- W2364458077 modified "2023-09-27" @default.
- W2364458077 title "RP-HPLC determination of tussilagone in Flos Farfarae" @default.
- W2364458077 hasPublicationYear "2009" @default.
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