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- W2364460259 abstract "Objective To explore the mitochondrial pathway in the apoptosis of Hela cells induced by TRAIL.Methods Apoptotic cells were detected by DNA electrophoresis.The mitochondria membrane potential(MMP),the expressions of BCL-2,the location of Cyt c and AIF,the caspase-3 activity were tested by confocal laser microscopy,western blot,immunofluorescence and caspase-3 activity assay.Results TRAIL can induce apoptosis in Hela cells.DNA ladders were showed on agarose gel electrophoresis.At the same time,TRAIL induced the decrease of MMP(P0.05,P0.01) and BCL-2,the release of Cyt c,the translocation of mitochondrial AIF to cytoplasm and nuclei,the increase of caspase-3 activity(the activity increased to 2.25±0.20 fold in 10 h treated cells,P0.05) in the time-dependent manner.Conclusions TRAIL induce the apoptosis of Hela cells through involves mitochondrial signal pathway." @default.
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- W2364460259 date "2011-01-01" @default.
- W2364460259 modified "2023-09-22" @default.
- W2364460259 title "TRAIL induced apoptosis in Hela cells by mitochondrial signal pathway" @default.
- W2364460259 hasPublicationYear "2011" @default.
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