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- W2364466085 abstract "Objective To investigate the feasibility of ABO genotype screening method using hair or mucous membrane in mouth and palatum as samples. Methods Discriminate the ABO genotypes of 8 samples of hair and 6 samples of mucous membrane in mouth and palatum by means of multi-polymerase chain reaction-restriction fragment length polymophism (multi PCR-RFLP). Amplify the samples' genomic DNA of the sample with two pairs of primers. Disgest the outcome of amplification with two restriction enzymes Msp Ⅰ and Kpn Ⅰ, then determine the result through fragment which appears after digestion. Afterward compare analysable results with ABO phenotypes determined by serological technique and genotypes of donors' blood samples. Results 6 ABO genotypes were found in 14 samples. The result is consistent on phenotypes and genotypes of blood samples. Conclusions This method is convenient, reprodueible and stable. It can discriminate 15 genotypes. Also it is applicable to forensic identification." @default.
- W2364466085 created "2016-06-24" @default.
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- W2364466085 date "2003-01-01" @default.
- W2364466085 modified "2023-09-23" @default.
- W2364466085 title "Discrimination of the ABO genotypes of hair and mucous membrane in mouth and palatum by means of multi PCR-RFLP" @default.
- W2364466085 hasPublicationYear "2003" @default.
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