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- W2364467306 abstract "Objective To optimize experimental condition of two dimensional gel electrophoresis(2-DE) technique for hippocampus. Methods Total protein samples were prepared from the isolated rat hippocampus and then determined by Bradford and separated by 2-DE.Comparisons were made on the effects of different sample preparations,including the pre-pH gradient strips(pH 3-10 NL,pH 4-7),300 μg and 700 μg protein sample volumes and the storage period of 2 months and 1 year. Results In present study,protein preparation of hippocampus from lysis buffer formulations and multiple high-speed cryogenic centrifuge were suitable for 2-DE.Isoelectric point(PI) of hippocampus protein mainly ranged from pH 4 to 8.Compared with that of 300 μg,700 μg protein sample volume was more ideal to control the signal to noise ratio(SNR).Fresh samples less affected 2-DE map with fewer gel stripes. Conclusion Ideal 2-DE map can be harvested with the hippocampus proteomics technique of using fresh sample,pH 4-7 gradient strips and 700 μg protein sample volume." @default.
- W2364467306 created "2016-06-24" @default.
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- W2364467306 date "2012-01-01" @default.
- W2364467306 modified "2023-09-23" @default.
- W2364467306 title "Optimization experimental condition of two dimensional gel electrophoresis technique for hippocampus" @default.
- W2364467306 hasPublicationYear "2012" @default.
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