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- W2364479539 abstract "Objective To investigate the effect of methylation transferase inhibitor 5-aza-2dC on the expression and methylation levels of Galectin-1 gene in human acutemyeloid leukemia(AML) cell line HL-60.Methods HL-60 cells were treated with 5-aza-2dC.Following the drug treatment,the expression of Galectin-1 was detected by real time reverse transcription-polymerase chain reaction(Real Time RT-PCR).The methylation levels of Galectin-1 gene were detected by methylation specific polymerase chain reaction(MSP).Results 5-aza-2dC increased the expression levels of Galectin-1 and decreased the methylation levels of Galectin-1 genes in HL-60 cells.Conclusions Demethylation of Galectin-1 genes could inverse its expression levels in HL-60 cells by treated with 5-aza-2Dc,which may be the anti-AML mechanism of 5-aza-2dC." @default.
- W2364479539 created "2016-06-24" @default.
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- W2364479539 date "2009-01-01" @default.
- W2364479539 modified "2023-09-23" @default.
- W2364479539 title "Effect of 5-aza-2dC on Expression and Methylation Levels of Galectin-1 Gene in Leukemia Cell Line HL-60" @default.
- W2364479539 hasPublicationYear "2009" @default.
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