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- W2364483805 abstract "Purpose To observe the stem cell factor(SCF) intervention on interstitial cells of Cajal-like cells(ICCs-like) c-kit mRNA and protein expression in the bladder of guinea pig in the high glucose environment.Methods The ICCs-like cells were isolated from the bladder of guinea pig by collagenase digestion and cultured for 24 h,then medium of 5 mmol/L(normal control group),15 mmol/L(high glucose) glucose concentration was added and continued to culture for 72 h.The high glucose group was divided into high glucose control group and SCF intervention group(50 ng/ml,100 ng/ml) and cultured for 24 h and 72 h.Last mRNA expression of c-kit was detected by reverse transcription polymerase chain reaction(RT-PCR) and protein expression of c-kit was tested and analyzed by laser scanning confocal microscope.Results Compared to control group,c-kit mRNA and protein expression levels of high glucose were significantly decreased.In 24 h group,c-kit mRNA and protein expression in SCF 50 ng/ml group did not increased significantly than high glucose control group(P0.05).However,c-kit mRNA and protein expression in SCF 100 ng/ml group were higher than that in high glucose control group(P0.05).In 72 h group,c-kit mRNA and protein expression in SCF50 ng/ml and 100 ng/ml group were higher than that in high glucose control group(P0.01).Conclusion High concentrations of SCF may increase the expression of c-kit in interstitial cells of Cajal which have changed in shape and function." @default.
- W2364483805 created "2016-06-24" @default.
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- W2364483805 date "2011-01-01" @default.
- W2364483805 modified "2023-09-24" @default.
- W2364483805 title "Effects of SCF on the expressions of c-kit mRNA and protein of interstitial cells of Cajal-like cells in the bladder of guinea pig in the high glucose environment" @default.
- W2364483805 hasPublicationYear "2011" @default.
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