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- W2364492872 abstract "Lipoxygenases are important enzymes in plant octadecanoic acid pathway.Products of this enzyme have important functions in plant growth and development,as well as in response to environmental stress.A prokaryotic expression vector of lipoxygenase gene from Adelostemma gracillimum was constructed through PCR and enzyme digestion connection method and a full length lipoxygenase cDNA was successfully expressed in E.coli BL21(DE3)under low temperature(15 ℃).Molecular weight of the recombinant protein was the same as the expected.Results of enzyme assay showed that the protein could catalyze linoleic acid to 9,10,11-trihydroxy-12-octadecenoic acid detected by LC-ESI-MS.It was speculated that octadecanoic acid pathway induced by chitosan differentiated to 9-LOX orientation." @default.
- W2364492872 created "2016-06-24" @default.
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- W2364492872 date "2012-01-01" @default.
- W2364492872 modified "2023-09-23" @default.
- W2364492872 title "Prokaryotic expression of lipoxygenase gene and crude enzyme activity assay from Adelostemma gracillimum" @default.
- W2364492872 hasPublicationYear "2012" @default.
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