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- W2364495420 abstract "In order to create an alternative treatment of phenylketonuria, recombinant expression plasmid pET28C-rPAL-1-cDNA with rice phenylalanine ammonia lyase(PAL) cDNA insert was constructed and its transformed E.coli BL21DE3 pET28C-rPAL-1-cDNA was established previously. Expression of the insert gene after isopropyl-β-D-thiogalactoside induction was demonstrated by SDS-PAGE and Mini-Protein two dimension electrophoresis. The molecular weight of the expressed fusion protein was estimated to be 78.6 ku, and was present mainly in the pellet(as the form of inclusion body) of the sonic disruptive bacteria. The specific activity of the pellet protein was 462 nmol·g -1·min -1 as measured by Zucker method and the K m of 0.50 mmol·L -1 and v max of 3.05 nmol·min -1 of the enzyme protein also estimated. Up to now, it was proved that the established E.coli BL21DE3 pET28C-rPAL-1-cDNA strain do express active PAL efficiently." @default.
- W2364495420 created "2016-06-24" @default.
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- W2364495420 date "2000-01-01" @default.
- W2364495420 modified "2023-09-26" @default.
- W2364495420 title "High-level expression of phenylalanine ammonia lyase with enzyme activity in E.coli by BL21DE3~( pET28C-rPAL-1-cDNA)" @default.
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