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- W2364499004 abstract "Objective:To study differentiation of human bone marrow derived mesenchymal stem cells (BDMSC) into heoatocytes for ideal cell origin of liver tissue engineering.Methods:After vary days of induction with hepatocyte growth factor(HGF) and fibroblast growth factor 4 (FGF-4) in 3D Matrigel, BDMSC and heoatocyte-like cells were determined by the utilization of morphological observation, immunofluorescence of alpha-fetoprotein(AFP) and albumin(Alb)and uptake and excretion of Indocyanine green(ICG) that were special for heoatocytes. Results:After 3D-cultured and induced with HGF and FGF-4 in vitro in Matrigel for 3-7 days, BDMSCs came into heoatocyte-like cells that expressed AFP and Alb and possessed ability of uptake and excretion of ICG.Conclusion:In vitro HGF、FGF and Matrigel maybe supply a proper microenviroment and play an important role during process of heoatocytes differentiation of BDMSC. This study offered a theoretical and technical foundation of mesenchymal stem cells (MSC) application for liver tissue engineering." @default.
- W2364499004 created "2016-06-24" @default.
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- W2364499004 date "2006-01-01" @default.
- W2364499004 modified "2023-09-25" @default.
- W2364499004 title "In vitro differentiation of human bone marrow derived mesenchymal stem cells into heoatocytes" @default.
- W2364499004 hasPublicationYear "2006" @default.
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