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- W2364502771 abstract "To clone and analyze the ribosomal DNA-ITS2(rDNA-ITS2) gene from Culex pipiens pallens in Shenzhen so as to provide molecular biology background for the precaution of mosquito vectors in breeding spot.By using the diagnostic primers for C.pipiens pallens,the sequence of the rDNA-ITS2 gene in the standard laboratory strains as well as the field-collected strains of mosquitoes were amplified by PCR.The amplified product was ligated to vector pGEM-Teasy,transformed to host bacteria DH5a and then the DNA sequence determination and analysis were performed accordingly.It was found that the rDNA-ITS2 gene was successfully cloned and the results on sequence analysis indicated that the sequences of rDNA-ITS2 gene of C.pipiens pallens both from the laboratory cloned and the breeding spot showed a homploque of more than 98% with that published in GenBank.Moreover,the existence of C.pipiens pallens in the breeding spot was also found.It is evident that the sequence of rDNA-ITS2 gene exhibits species-specificity,thus it can provide a molecular biological basis for the precaution of mosquito vector in the breeding spot." @default.
- W2364502771 created "2016-06-24" @default.
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- W2364502771 date "2007-01-01" @default.
- W2364502771 modified "2023-09-23" @default.
- W2364502771 title "Cloning and analysis of the ribosomal DNA-ITS2 gene in Culex pipiens pallens" @default.
- W2364502771 hasPublicationYear "2007" @default.
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