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- W2364505972 abstract "Objective:To evaluate the performance of alkaline phosphatase(AP)-tagging technique in functional study ofeukaryotic genes and study of the axon guidance activity and receptors of netrin-4.Methods:The cDNA sequence codingnetrin-4 and the three domains were subcloned into the alkaline phosphatase vector APtag4 to allowsuccessfull expression ofthe alkaline phosphatase tagged proteins in COS7 cells.The activity of netrin-4 was identified using cultured neuronal ex-plants and neurons.Results:The netrin4 had axon guidance function.The AP4-netrin4 fusion protein was also found to beable to bind to the primary cultured neuron by examining the activity of AP tag.The AP4-LNTand AP4-NCTwere able tobind to the primary cultured neuron,but AP4-EGFL and AP4 alone not.Conclusion:There are receptors for netrin-4 inthe surface of primary cultured neuron,and the key binding domains are LNT and NCT.The strategy using AP4-tag is apowerful technique for functional study of eukaryotic genes." @default.
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- W2364505972 date "2006-01-01" @default.
- W2364505972 modified "2023-09-25" @default.
- W2364505972 title "Application of alkaline phosphatase tag in functional study of axon guidance factor netrin-4" @default.
- W2364505972 hasPublicationYear "2006" @default.
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