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- W2364506144 abstract "Organochlorine pesticide 1,1,1-trichloro-2,2-bis-(p-chlorophenyl)ethane(DDT) is a persistent and broad toxic organochlorine insecticide. DDT is slowly degraded in tissues mainly to 2,2-bis(4-chlorophenyl)acetic acid(DDA). DDT and its decomposition products are ubiquitous micropollutants in seawater and shellfish. This study presents a novel method of preparing the polyclonal antibody against DDA to investigate the levels of DDT and DDA in seawater and shellfish using indirect competitive enzyme-linked immunosorbent assay (idc-ELISA). Under the presence of N,N-dicyclohexylcarbodiimide, the complete antigen can be obtained by coupling the hapten (DDA) with bovine serum albumin (BSA) and ovalbumin (OVA), respectively. The female BALB/c mice were immunized using DDA-BSA as immunogen to prepare the polyclonal antibody against DDA and its characterization was studied. The results indicated that the antibodies produced from five mice immunized possessed highly sensitive and specific immune reaction for DDA. Using DDA-OVA as coating antigen, the 5# antibody was employed to develop the idc-ELISA method to investigate the concentrations of DDA in seawater and shellfish. The limit of detection (LOD) of the method was 10.0 ng·mL-1. The average recovery and coefficient of variation (CV) were 122% and 30% for seawater, and 131% and 22% for shellfish, respectively." @default.
- W2364506144 created "2016-06-24" @default.
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- W2364506144 date "2008-01-01" @default.
- W2364506144 modified "2023-09-28" @default.
- W2364506144 title "Preparation and Caracterization of Polyclonal Antibody Against DDA" @default.
- W2364506144 hasPublicationYear "2008" @default.
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