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- W2364506549 abstract "Aim To study the biocompatibility of recombinant human-like collagenⅡ(RHLCⅡ)membranes crosslinked with glutaraldehyde.Methods RHLCⅡwas crosslinked with different concentrations(0.005 mol/L and 0.01 mol/L) of glutaraldehyde to form two groups of RHLCⅡ membranes.Fibroblasts were cultured as seeding cells on RHLCⅡ membranes,then the cells-material complex was observed with contrast and scanning electronic microscope.The proliferation of fibroblasts were tested by MTT.RHLCⅡ membranes′ cytotoxicity was evaluated.Results After 6 h cultivation,fibroblasts adhered to both groups.Fibroblasts formed several confluent layers on two groups of RHLC membranes in 96 h.Fibroblasts can proliferate on two kinds of RHLCⅡ membranes within 96 h.Compared with RHLCⅡ membranes crosslinked by 0.005 mol/L glutaraldehyde,RHLCⅡ membranes with 0.01 mol/L glutaraldehyde have stronger cytotoxicity.But reaction scales of both RHLCⅡ membranes 0-1 level.Cytotoxicity of RHLCⅡ membranes met the requirement of standards.Conclusion RHLCⅡ membranes crosslinked with 0.005 mol/L and 0.01 mol/L glutaraldehyde have good biocompatibility and can become ideal materials for skin tissue engineering." @default.
- W2364506549 created "2016-06-24" @default.
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- W2364506549 date "2007-01-01" @default.
- W2364506549 modified "2023-09-22" @default.
- W2364506549 title "Biocompatibility of recombinant human-like collagen II membranes crosslinked with glutaraldehyde" @default.
- W2364506549 hasPublicationYear "2007" @default.
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