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- W2364508943 abstract "Gonadotropin-releasing hormone(GnRH) is a conserved nerval decapeptide that regulates the development and maintenance of reproductive function in vertebrate.The cDNA encoding the [His5Trp7Tyr8] gonadotropin-releasing hormone(chicken-Ⅱ-type GnRH,cGnRH-Ⅱ) precursor from the brain of rice field eel(Monopterus albus) has been isolated and sequenced by using reverse transcription and rapid amplification of cDNA ends(RACE).From the subcloning and sequence analysis of 3′-RACE and 5′-RACE product,the complete sequence of the cDNA encoding its cGnRH-Ⅱpeptide was identified and determined to be 617 bp in length.The structure of the encoded peptide is identical to all other cGnRH-Ⅱ reported to date.The deduced peptide is composed of a signal peptide(SP),a GnRH decapeptide and a GnRH-associated peptide(GAP) which is connected to GnRH by a Gly-Lys-Arg sequence.The cGnRH-Ⅱ cDNA encoded SP of 21 amino acids and GAP of 49 amino acids.The amino acid sequence of cGnRHⅡ in combining with the Gly-Lys-Arg sequence is highly conserved during evolution when compared with the corresponding regions of other GnRH precursors.However,the untranslated region of GnRH are markedly divergent in nucleotide sequences." @default.
- W2364508943 created "2016-06-24" @default.
- W2364508943 creator A5020086934 @default.
- W2364508943 date "2007-01-01" @default.
- W2364508943 modified "2023-09-25" @default.
- W2364508943 title "CLONING AND SEQUENCE ANALYSIS OF cDNA ENCODING cGnRH-II FROM RICE FIELD EEL(MONOPTERUS ALBUS)" @default.
- W2364508943 hasPublicationYear "2007" @default.
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