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- W2364523360 abstract "To obtain recombinant secretive BACE1 proteins, study its function and further look for its specific inhibitor, the ectodomain fragment of human BACE1 coding region from human fetal hippocampal tissue is amplified using RT-PCR method. Confirmed by sequencing, clone the target gene into a pFastBac donor plasmid. After the recombinant pFastBac-BACE1-454 had been determined to be correct, the pFast-Bac-BACE1-454 was transformed into DH10Bac competent cells for transposition into a baculovirus shuttle vector Bacmid. Using agarose gel electrophoresis and PCR analysis to verify successful transposition. Recombinant Bacmid-BACE1-454 was transfected into Sf9 cells with CellFECTIN(?) Reagent and harvest virus from culture medium. Recombinant baculovirus particles were infected into insect cells and express the BACE1-454 protein. These expressed BACE1-454 proteins were identified by immunoblot with anti-BACE monoclonal antibody." @default.
- W2364523360 created "2016-06-24" @default.
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- W2364523360 date "2005-01-01" @default.
- W2364523360 modified "2023-09-27" @default.
- W2364523360 title "Eukaryotic Expression of Soluble BACE1 in Insect Cells" @default.
- W2364523360 hasPublicationYear "2005" @default.
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