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- W2364530281 abstract "Purpose The aim of this study was to reveal the role of SGT,the small glutamine-rich tetratricopeptide repeat(TPR)-containing protein,in the developing mouse brain through examining the expression profile of SGT during the development stage.Methods In this study,quantitative RT-RCR and Western blot were applied to investigate the expression of SGT mRNA and protein in the mouse whole brain.Western bolt was also used to detect the expression of SGT in the hippocampus,cerebral cortex,striatum and cerebellum.Immunohistochemical analysis on postnatal and adult mouse brain was performed to examine the subcellular localization of SGT.Results Our data showed that the levels of SGT mRNA and protein in the mouse whole brain were both high during the postnatal stage and declined in the adult.Regional expression of SGT protein in the hippocampus,cerebral cortex,striatum and cerebellum showed a similar expression profile.Immunohistochemical analysis found that in the P14 mouse brain,SGT was abundant in all the CA regions of hippocampus as well as most regions of cerebral cortex and striatum.In the cerebellum,SGT was mainly distributed in Purkinje cells.In the sections of the adult mouse brain,faint expression was observed in the regions mentioned above.Conclusions Our findings firstly exhibit the expression pattern of SGT in the mouse brain development,which might shed new light on further functional analysis of SGT in the central nervous system." @default.
- W2364530281 created "2016-06-24" @default.
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- W2364530281 date "2007-01-01" @default.
- W2364530281 modified "2023-09-24" @default.
- W2364530281 title "Expression of small glutamine-rich TPR-containing protein in the developing mouse brain" @default.
- W2364530281 hasPublicationYear "2007" @default.
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