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- W2364559962 abstract "Objective To observe the effects of hyaluronic acid which inhibits macrophage migration inhibitory factor on the expression of Bcl-2 and p53 protein in human gastric cancer cell line BGC-823 in vitro. Methods Reverse transcriptase polymerase chain reaction(RT-PCR) was used to detect the effects of HA on the expression of macrophage migration inhibitory factor(MIF) mRNA in BGC-823 cells;Western blot was used to detect the effects of HA on the expression of the protein of MIF and apoptotic related gene protein Bcl-2 and p53 in BGC-823 cells. Results The result of RT-PCR showed that the relative expression level of MIF mRNA in BGC-823 cells was decreased with higher concentration and longer time which shows a concentration-and time-dependent manner.The result of Western blot showed that the relative expression levels of MIF and Bcl-2 protein in BGC-823 cells were decreased with higher concentration or longer time,but the relative expression level of p53 protein was increased with higher concentration or longer time which shows a concentration-and time-dependent manner. Conclusions MIF can regulate the expression of Bcl-2 and p53 protein in human gastric cancer cells." @default.
- W2364559962 created "2016-06-24" @default.
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- W2364559962 date "2009-01-01" @default.
- W2364559962 modified "2023-09-23" @default.
- W2364559962 title "Effects of Macrophage Migration Inhibitory Factor Inhibitor HA on the Expressions of Bcl-2 and p53 Protein in Human Gastric Cancer Cell Line BGC-823" @default.
- W2364559962 hasPublicationYear "2009" @default.
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