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- W2364565837 abstract "Objective To study the effect of water soluble azone on the viability and ultrastructure of rabbit lens epithelial cells cultured in vitro in order to probe into the safety application concentration of the drug for the eye in the clinical practice. Methods Rabbit lens epithelial cells were cultured in vitro.The cells were exposed to aqueous solution of azone in concentrations of 0.001,0.005,0.010,0.050,0.100,0.500 and 1.000 g·L-1,respectively.Viability and ultrastructure of the cells were then assayed with cell counting,MTT method and scanning as well as transmission electron microscopy,respectively. Results Viability of the rabbit lens epithelial cells was not affected after they had been exposed to water soluble azone in concentrations lower than 0.10 g·L-1 for 24 h.A decline in the viability of cells was noticed after a 24 h exposure of cells to water soluble azone in concentrations higher than 0.500 g·L-1(P0.01).Organelles of the lens epithelial cells were not injured after a 10 minute exposure of the cells to water soluble azone in concentrations lower than 0.100 g·L-1,as evidenced under the transmission electron microscope.Water soluble azone in concentrations ≥0.500 g·L-1,however,was shown to cause extensive cell death. Conclusion Water soluble azone in concentrations no higher than 0.100 g·L-1 are unlikely to cause decline in viability of and injury of organelles in lens epithelial cells.Aqueous solution of azone in these concentrations,therefore,is of considerable application value to the clinical practice." @default.
- W2364565837 created "2016-06-24" @default.
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- W2364565837 date "2007-01-01" @default.
- W2364565837 modified "2023-09-24" @default.
- W2364565837 title "Effect of Water Soluble Azone on the Viability and Ultrastructure of Rabbit Lens Epithelial Cells" @default.
- W2364565837 hasPublicationYear "2007" @default.
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