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- W2364567652 abstract "Objective To study the effect of basic fibroblast growth factor(b FGF) on proliferation and chondrogenetic potency of porcine elastic chondrocytes in vitro.Methods Cells were harvested from the elastic auricular cartilage of swines.Viability and quantification of the cells were determined.Cells were plated at equal concentration in different plates.The effect of b FGF on cell growth and chondrogenetic potency was studied in the following groups:Group 1(control group) receiving Ham's F 12 with supplements but no b FGF;Group 2 receiving 10?μg/L b FGF;Group 3 receiving 25?μg/L b FGF;Group 4 receiving 50?μg/L b FGF.Results Chondrocytes displayed a morphology similar to fibroblasts in the media containing b FGF.After 3 day culture these cells returned to the original shape;Compared to the group 1,all of the other groups receiving b FGF approximately had a 70 fold increase in total cell number at the third passage;The cells in the third passage were still able to produce type Ⅱ collagen demonstrated by immunohistochemistry and in situ hybridization;The quantity of GAG secreted by cells in all 4 groups had no difference.Conclusions Chondrocytes can be vastly expanded from a small number of cells without losing their phenotype when b FGF was added,indicating that b FGF offers the possibility to provide large amounts of seed cells which can be expanded from small amount of harvested tissue.Among the three different concentrations of b FGF,the optimal concentration of b FGF is 10?μg/L." @default.
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- W2364567652 date "2002-01-01" @default.
- W2364567652 modified "2023-09-27" @default.
- W2364567652 title "The effect of basic fibroblast growth factor on proliferation and chondrogenetic potency of porcine elastic chondrocytes in vitro" @default.
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