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- W2364581608 abstract "Objective To establish real-time fluorescence quantitation polymerase chain reaction(PCR) for the detection and epidemiological studies of Streptococcus pneumoniae.Methods The autolysin(lyt) gene and hemolysin(ply) gene sequences were selected to develop primers and probe.A total of 10 strains of Streptococcus pneumoniae,13 strains of non-Streptococcus pneumoniae DNA and the different concentration strains of Streptococcus pneumoniae DNA were detected by real-time fluorescence quantitation PCR,and 200 clinical specimens were detected by primers and probe.The specificity and sensitivity were also analyzed.Results The 10 strains of Streptococcus pneumoniae were measured to obtain amplification products.However,13 strains of non-Streptococcus pneumoniae DNA had no obvious amplification,and the sensitivity was 100fg.Among the 200 clinical specimens,real-time fluorescence quantitation PCR detected 42 cases of Streptococcus pneumoniae-positive(the positive rate was 21%),while the culture method detected 16 cases of Streptococcus pneumoniae-positive(the positive rate was 8%).Conclusions Real-time fluorescence quantitation PCR is a rapid,sensitive and specific assay for the detection of Streptococcus pneumoniae.It can be used for the diagnosis and epidemiological studies." @default.
- W2364581608 created "2016-06-24" @default.
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- W2364581608 date "2013-01-01" @default.
- W2364581608 modified "2023-09-23" @default.
- W2364581608 title "Rapid detection of Streptococcus pneumoniae by real-time fluorescence quantitation PCR" @default.
- W2364581608 hasPublicationYear "2013" @default.
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